A clearing-free protocol for imaging intact whole adipose tissue innervation in mice.
STAR Protoc
; 3(1): 101109, 2022 03 18.
Article
em En
| MEDLINE
| ID: mdl-35106499
ABSTRACT
Here we provide a clearing-free protocol for processing intact, whole mount subcutaneous white adipose tissue (scWAT) for immunofluorescence as an alternative to current clearing-based approaches. We use a combination of Z-depth reduction and autofluorescence quenching techniques to fluorescently label, image, and quantify adipose tissue innervation effectively throughout intact mouse tissues without the need for optical clearing or light sheet microscopy. This protocol has been optimized and validated for adipose neurovascular labeling. For complete details on the use and execution of this protocol, please refer to Willows et al. (2021).
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Texto completo:
1
Base de dados:
MEDLINE
Assunto principal:
Tecido Adiposo
/
Imageamento Tridimensional
Limite:
Animals
Idioma:
En
Ano de publicação:
2022
Tipo de documento:
Article