Sustained Release Biodegradable Microneedles of Difluprednate for Delivery to Posterior Eye.

ABSTRACT

Purpose: Difluprednate ophthalmic emulsion (Durezol®) is currently used for the treatment of anterior uveitis; however, recent studies have shown that difluprednate can treat posterior eye conditions. Topical formulations limit the amount of drug capable of permeating to the posterior segment due to permeation barriers, lacrimation, and lymphatic clearance. Methods: Resomer®-based microneedle patches were fabricated for difluprednate using poly(acrylic acid) (PAA) for the rapidly dissolvable backing. The patches were analyzed for microneedle uniformity and sharpness using scanning electron microscopy, and the penetration depth was analyzed by confocal microscopy. Failure force necessary to break the microneedles and force needed to penetrate the sclera were analyzed by the texture analyzer. Difluprednate release and trans-scleral permeation studies on microneedles were performed using Franz diffusion cells. Results: The microneedles were uniform, sharp, and penetrated to 500 µm depth on sclera. The microneedles have a failure force proportional to the molecular weight (MW) of the polymer used. There was no correlation between failure force and the penetration force of the microneedles. The PAA backing dissolved within 30-40 min, while release studies showed a matrix diffusion-controlled release over the 7-day study. The amount of drug permeation and retention in the sclera were decreased with an increase in the MW of the Resomer and failure force of each array. Conclusions: Resomer-based microneedles have a potential application for the sustained release of difluprednate for posterior segment conditions.