[~1H-qNMR quantification of total ginsenosides in Shenmai Injection].
Zhongguo Zhong Yao Za Zhi
; 47(3): 587-592, 2022 Feb.
Article
em Zh
| MEDLINE
| ID: mdl-35178940
ABSTRACT
A content determination method based on ~1H-qNMR was developed for the determination of total ginsenosides in Shenmai Injection. The parameters were optimized with CD_3OD as the solvent, dimethyl terephthalate as the internal standard, the peak at δ 8.11 as the internal standard peak, and the peaks at δ 1.68 and δ 0.79 as quantitative peaks of total ginsenosides. The developed ~1H-qNMR-based method was validated methodologically. The results showed that the method could achieve accurate measurement of total ginsenosides in Shenmai Injection in the range of 0.167 6-3.091 1 mmol·L~(-1). The developed ~1H-qNMR-based method for total ginsenosides is simple in operation, short in analysis time, strong in specificity, independent of accompanying standard curve, and small in sample volume, which can serve as a reliable mean for the quality control of Shenmai Injection. This study is expected to provide new ideas for the development of quantification methods of total ginsenosides.
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Base de dados:
MEDLINE
Assunto principal:
Medicamentos de Ervas Chinesas
/
Ginsenosídeos
Idioma:
Zh
Ano de publicação:
2022
Tipo de documento:
Article