Mouse Model for Optogenetic Genome Engineering.
Acta Med Okayama
; 76(1): 1-5, 2022 Feb.
Article
em En
| MEDLINE
| ID: mdl-35236992
ABSTRACT
Optogenetics, a technology to manipulate biological phenomena thorough light, has attracted much attention in neuroscience. Recently, the Magnet System, a photo-inducible protein dimerization system which can control the intracellular behavior of various biomolecules with high accuracy using light was developed. Furthermore, photoactivation systems for controlling biological phenomena are being developed by combining this technique with genome-editing technology (CRISPR/Cas9 System) or DNA recombination technology (Cre-loxP system). Herein, we review the history of optogenetics and the latest Magnet System technology and introduce our recently developed photoactivatable Cre knock-in mice with temporal-, spatial-, and cell-specific accuracy.
Palavras-chave
Texto completo:
1
Base de dados:
MEDLINE
Assunto principal:
Engenharia Genética
/
Modelos Animais de Doenças
/
Optogenética
Tipo de estudo:
Prognostic_studies
Limite:
Animals
Idioma:
En
Ano de publicação:
2022
Tipo de documento:
Article