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Landscape of adenosine-to-inosine RNA recoding across human tissues.
Gabay, Orshay; Shoshan, Yoav; Kopel, Eli; Ben-Zvi, Udi; Mann, Tomer D; Bressler, Noam; Cohen-Fultheim, Roni; Schaffer, Amos A; Roth, Shalom Hillel; Tzur, Ziv; Levanon, Erez Y; Eisenberg, Eli.
Afiliação
  • Gabay O; The Mina and Everard Goodman Faculty of Life Sciences, Bar-Ilan University, Ramat Gan, 5290002, Israel.
  • Shoshan Y; Raymond and Beverly Sackler School of Physics and Astronomy and Sagol School of Neuroscience, Tel Aviv University, Tel Aviv, 6997801, Israel.
  • Kopel E; The Mina and Everard Goodman Faculty of Life Sciences, Bar-Ilan University, Ramat Gan, 5290002, Israel.
  • Ben-Zvi U; The Mina and Everard Goodman Faculty of Life Sciences, Bar-Ilan University, Ramat Gan, 5290002, Israel.
  • Mann TD; Tel Aviv Sourasky Medical Center and Sackler school of medicine, Tel Aviv University, Tel Aviv, Israel.
  • Bressler N; Raymond and Beverly Sackler School of Physics and Astronomy and Sagol School of Neuroscience, Tel Aviv University, Tel Aviv, 6997801, Israel.
  • Cohen-Fultheim R; The Mina and Everard Goodman Faculty of Life Sciences, Bar-Ilan University, Ramat Gan, 5290002, Israel.
  • Schaffer AA; The Mina and Everard Goodman Faculty of Life Sciences, Bar-Ilan University, Ramat Gan, 5290002, Israel.
  • Roth SH; The Mina and Everard Goodman Faculty of Life Sciences, Bar-Ilan University, Ramat Gan, 5290002, Israel.
  • Tzur Z; The Mina and Everard Goodman Faculty of Life Sciences, Bar-Ilan University, Ramat Gan, 5290002, Israel.
  • Levanon EY; The Mina and Everard Goodman Faculty of Life Sciences, Bar-Ilan University, Ramat Gan, 5290002, Israel. erez.levanon@biu.ac.il.
  • Eisenberg E; The Institute of Nanotechnology and Advanced Materials, Bar-Ilan University, Ramat Gan, 5290002, Israel. erez.levanon@biu.ac.il.
Nat Commun ; 13(1): 1184, 2022 03 04.
Article em En | MEDLINE | ID: mdl-35246538
ABSTRACT
RNA editing by adenosine deaminases changes the information encoded in the mRNA from its genomic blueprint. Editing of protein-coding sequences can introduce novel, functionally distinct, protein isoforms and diversify the proteome. The functional importance of a few recoding sites has been appreciated for decades. However, systematic methods to uncover these sites perform poorly, and the full repertoire of recoding in human and other mammals is unknown. Here we present a new detection approach, and analyze 9125 GTEx RNA-seq samples, to produce a highly-accurate atlas of 1517 editing sites within the coding region and their editing levels across human tissues. Single-cell RNA-seq data shows protein recoding contributes to the variability across cell subpopulations. Most highly edited sites are evolutionary conserved in non-primate mammals, attesting for adaptation. This comprehensive set can facilitate understanding of the role of recoding in human physiology and diseases.
Assuntos

Texto completo: 1 Base de dados: MEDLINE Assunto principal: RNA / Adenosina Limite: Animals / Humans Idioma: En Ano de publicação: 2022 Tipo de documento: Article

Texto completo: 1 Base de dados: MEDLINE Assunto principal: RNA / Adenosina Limite: Animals / Humans Idioma: En Ano de publicação: 2022 Tipo de documento: Article