Your browser doesn't support javascript.
loading
Continuous ZnO nanoparticle exposure induces melanoma-like skin lesions in epidermal barrier dysfunction model mice through anti-apoptotic effects mediated by the oxidative stress-activated NF-κB pathway.
Wang, Ping; Hu, Guodong; Zhao, Wen; Du, Juan; You, Menghan; Xv, Mengying; Yang, Hong; Zhang, Min; Yan, Fang; Huang, Mianbo; Wang, Xueer; Zhang, Lin; Chen, Yinghua.
Afiliação
  • Wang P; Department of Histology and Embryology, Guangdong Provincial Key Laboratory of Construction and Detection in Tissue Engineering, NMPA Key Laboratory for Safety Evaluation of Cosmetics, National Demonstration Center for Experimental Education, School of Basic Medical Sciences, Southern Medical Univer
  • Hu G; Department of Respiratory and Critical Care Medicine, Nanfang Hospital, Southern Medical University, Guangzhou, 510515, China.
  • Zhao W; Department of Medical Imaging, Guangzhou Women and Children's Medical Center, National Children's Medical Center for South Central Region, Guangzhou, 510515, China.
  • Du J; Department of Inspection and Quarantine (Hygiene Detection Center), School of Public Health, Southern Medical University, Guangzhou, 510515, China.
  • You M; Department of Histology and Embryology, Guangdong Provincial Key Laboratory of Construction and Detection in Tissue Engineering, NMPA Key Laboratory for Safety Evaluation of Cosmetics, National Demonstration Center for Experimental Education, School of Basic Medical Sciences, Southern Medical Univer
  • Xv M; Department of Respiratory and Critical Care Medicine, The People's Hospital of Yangjiang, Yangjiang, 529500, China.
  • Yang H; Department of Respiratory and Critical Care Medicine, Nanfang Hospital, Southern Medical University, Guangzhou, 510515, China.
  • Zhang M; Department of Histology and Embryology, Guangdong Provincial Key Laboratory of Construction and Detection in Tissue Engineering, NMPA Key Laboratory for Safety Evaluation of Cosmetics, National Demonstration Center for Experimental Education, School of Basic Medical Sciences, Southern Medical Univer
  • Yan F; Department of Histology and Embryology, Guangdong Provincial Key Laboratory of Construction and Detection in Tissue Engineering, NMPA Key Laboratory for Safety Evaluation of Cosmetics, National Demonstration Center for Experimental Education, School of Basic Medical Sciences, Southern Medical Univer
  • Huang M; Department of Histology and Embryology, Guangdong Provincial Key Laboratory of Construction and Detection in Tissue Engineering, NMPA Key Laboratory for Safety Evaluation of Cosmetics, National Demonstration Center for Experimental Education, School of Basic Medical Sciences, Southern Medical Univer
  • Wang X; Department of Histology and Embryology, Guangdong Provincial Key Laboratory of Construction and Detection in Tissue Engineering, NMPA Key Laboratory for Safety Evaluation of Cosmetics, National Demonstration Center for Experimental Education, School of Basic Medical Sciences, Southern Medical Univer
  • Zhang L; Department of Histology and Embryology, Guangdong Provincial Key Laboratory of Construction and Detection in Tissue Engineering, NMPA Key Laboratory for Safety Evaluation of Cosmetics, National Demonstration Center for Experimental Education, School of Basic Medical Sciences, Southern Medical Univer
  • Chen Y; Department of Histology and Embryology, Guangdong Provincial Key Laboratory of Construction and Detection in Tissue Engineering, NMPA Key Laboratory for Safety Evaluation of Cosmetics, National Demonstration Center for Experimental Education, School of Basic Medical Sciences, Southern Medical Univer
J Nanobiotechnology ; 20(1): 111, 2022 Mar 05.
Article em En | MEDLINE | ID: mdl-35248056
BACKGROUND: Increasing interest in the hazardous properties of zinc oxide nanoparticles (ZnO NPs), commonly used as ultraviolet filters in sunscreen, has driven efforts to study the percutaneous application of ZnO NPs to diseased skin; however, in-depth studies of toxic effects on melanocytes under conditions of epidermal barrier dysfunction remain lacking. METHODS: Epidermal barrier dysfunction model mice were continuously exposed to a ZnO NP-containing suspension for 14 and 49 consecutive days in vivo. Melanoma-like change and molecular mechanisms were also verified in human epidermal melanocytes treated with 5.0 µg/ml ZnO NPs for 72 h in vitro. RESULTS: ZnO NP application for 14 and 49 consecutive days induced melanoma-like skin lesions, supported by pigmented appearance, markedly increased number of melanocytes in the epidermis and dermis, increased cells with irregular nuclei in the epidermis, recruited dendritic cells in the dermis and dysregulated expression of melanoma-associated gene Fkbp51, Trim63 and Tsp 1. ZnO NPs increased oxidative injury, inhibited apoptosis, and increased nuclear factor kappa B (NF-κB) p65 and Bcl-2 expression in melanocytes of skin with epidermal barrier dysfunction after continuously treated for 14 and 49 days. Exposure to 5.0 µg/ml ZnO NPs for 72 h increased cell viability, decreased apoptosis, and increased Fkbp51 expression in melanocytes, consistent with histological observations in vivo. The oxidative stress-mediated mechanism underlying the induction of anti-apoptotic effects was verified using the reactive oxygen species scavenger N-acetylcysteine. CONCLUSIONS: The entry of ZnO NPs into the stratum basale of skin with epidermal barrier dysfunction resulted in melanoma-like skin lesions and an anti-apoptotic effect induced by oxidative stress, activating the NF-κB pathway in melanocytes.
Assuntos
Palavras-chave

Texto completo: 1 Base de dados: MEDLINE Assunto principal: Óxido de Zinco / Nanopartículas / Melanoma Tipo de estudo: Prognostic_studies Limite: Animals Idioma: En Ano de publicação: 2022 Tipo de documento: Article

Texto completo: 1 Base de dados: MEDLINE Assunto principal: Óxido de Zinco / Nanopartículas / Melanoma Tipo de estudo: Prognostic_studies Limite: Animals Idioma: En Ano de publicação: 2022 Tipo de documento: Article