Binary MoS2 nanostructures as nanocarriers for amplification in multiplexed electrochemical immunosensing: simultaneous determination of B cell activation factor and proliferation-induced signal immunity-related cytokines.
Mikrochim Acta
; 189(4): 143, 2022 03 14.
Article
em En
| MEDLINE
| ID: mdl-35286499
ABSTRACT
A dual immunosensor is reported for the simultaneous determination of two important immunity-related cytokines BAFF (B cell activation factor) and APRIL (a proliferation-induced signal). Sandwich-type immunoassays with specific antibodies (cAbs) and a strategy for signal amplification based on labelling the detection antibodies (dAbs) with binary MoS2/MWCNTs nanostructures and using horseradish peroxidase (HRP) were implemented. Amperometric detection was carried out at screen-printed dual carbon electrodes (SPdCEs) through the hydroquinone HQ/H2O2 system. The developed dual immunosensor provided limit of detection (LOD) of 0.08 and 0.06 ng mL-1 for BAFF and APRIL, respectively, and proved to be useful for the determination of both cytokines in cancer cell lysates and serum samples from patients diagnosed with autoimmune diseases and cancer. The obtained results agreed with those found using ELISA methodologies.
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Base de dados:
MEDLINE
Assunto principal:
Técnicas Biossensoriais
/
Nanoestruturas
Limite:
Humans
Idioma:
En
Ano de publicação:
2022
Tipo de documento:
Article