Assessing DNA Damage Responses Using B Lymphocyte Cultures.
Methods Mol Biol
; 2444: 69-80, 2022.
Article
em En
| MEDLINE
| ID: mdl-35290632
ABSTRACT
Development of B cells requires the programmed generation and repair of double-stranded DNA breaks in antigen receptor genes. Investigation of the cellular responses to these DNA breaks has established important insights into B cell development and, more broadly, has provided fundamental advances into the molecular mechanisms of DNA damage response pathways. Abelson transformed pre-B cell lines and primary pre-B cell cultures are malleable experimental systems with diverse applications for studying DNA damage responses. This chapter describes methods for generating these cellular systems, inducing and quantifying DSBs, and assessing DNA damage programs.
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Texto completo:
1
Base de dados:
MEDLINE
Assunto principal:
Linfoma de Células B
/
Quebras de DNA de Cadeia Dupla
Limite:
Humans
Idioma:
En
Ano de publicação:
2022
Tipo de documento:
Article