Identification of salivary gland escape barriers to western equine encephalitis virus in the natural vector, Culex tarsalis.
PLoS One
; 17(3): e0262967, 2022.
Article
em En
| MEDLINE
| ID: mdl-35298486
ABSTRACT
Herein we describe a previously uninvestigated salivary gland escape barrier (SEB) in Culex tarsalis mosquitoes infected with two different strains of Western equine encephalitis virus (WEEV). The WEEV strains were originally isolated either from mosquitoes (IMP181) or a human patient (McMillan). Both IMP181 and McMillan viruses were fully able to infect the salivary glands of Culex tarsalis after intrathoracic injection as determined by expression of mCherry fluorescent protein. IMP181, however, was better adapted to transmission as measured by virus titer in saliva as well as transmission rates in infected mosquitoes. We used chimeric recombinant WEEV strains to show that inclusion of IMP181-derived structural genes partially circumvents the SEB.
Texto completo:
1
Base de dados:
MEDLINE
Assunto principal:
Culex
/
Encefalomielite Equina
Tipo de estudo:
Diagnostic_studies
Limite:
Animals
/
Humans
Idioma:
En
Ano de publicação:
2022
Tipo de documento:
Article