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Variable penetrance of Nab3 granule accumulation quantified by a new tool for high-throughput single-cell granule analysis.
Hunn, Jeremy C; Hutchinson, Katherine M; Kelley, Joshua B; Reines, Daniel.
Afiliação
  • Hunn JC; Department of Biochemistry, Emory University School of Medicine, Atlanta, GA, USA.
  • Hutchinson KM; Department of Biochemistry, Emory University School of Medicine, Atlanta, GA, USA.
  • Kelley JB; Department of Molecular Biosciences, University of Maine, Orono, ME, USA.
  • Reines D; Department of Biochemistry, Emory University School of Medicine, Atlanta, GA, USA. dreines@emory.edu.
Curr Genet ; 68(3-4): 467-480, 2022 Aug.
Article em En | MEDLINE | ID: mdl-35301575
Reorganization of cellular proteins into subcellular compartments, such as the concentration of RNA-binding proteins into cytoplasmic stress granules and P-bodies, is a well-recognized, widely studied physiological process currently under intense investigation. One example of this is the induction of the yeast Nab3 transcription termination factor to rearrange from its pan-nucleoplasmic distribution to a granule at the nuclear periphery in response to nutrient limitation. Recent work in many cell types has shown that protein condensation in the nucleus is functionally important for transcription initiation, RNA processing, and termination. However, little is known about how subnuclear compartments form. Here, we have quantitatively analyzed this dynamic process in living yeast using a high-throughput computational tool and fluorescence microscopy. This analysis revealed that Nab3 granule accumulation varies in penetrance across yeast strains. A concentrated single granule is formed from at least a quarter of the nuclear Nab3 drawn from the rest of the nucleus. Levels of granule accumulation were inversely correlated with a growth defect in the absence of glucose. Importantly, the basis for some of the variation in penetrance was attributable to a defect in mitochondrial function. This publicly available computational tool provides a rigorous, reproducible, and unbiased examination of Nab3 granule accumulation that should be widely applicable to a variety of fluorescent images. Thousands of live cells can be readily examined enabling rigorous statistical verification of significance. With it, we describe a new feature of inducible subnuclear compartment formation for RNA-binding transcription factors and an important determinant of granule biogenesis.
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Texto completo: 1 Base de dados: MEDLINE Assunto principal: Saccharomyces cerevisiae / Proteínas de Saccharomyces cerevisiae Idioma: En Ano de publicação: 2022 Tipo de documento: Article

Texto completo: 1 Base de dados: MEDLINE Assunto principal: Saccharomyces cerevisiae / Proteínas de Saccharomyces cerevisiae Idioma: En Ano de publicação: 2022 Tipo de documento: Article