Solution Chemistry of Dihydroxyacetone and Synthesis of Monomeric Dihydroxyacetone.
Chem Res Toxicol
; 35(4): 616-625, 2022 04 18.
Article
em En
| MEDLINE
| ID: mdl-35324152
ABSTRACT
Dihydroxyacetone (DHA) is a major byproduct of e-cigarette combustion and is the active ingredient in sunless tanning products. Mounting evidence points to its damaging effects on cellular functions. While developing a simple synthetic route to monomeric [13C3]DHA for flux metabolic studies that compared DHA and glyceraldehyde (GA) metabolism, we uncovered that solid DHA ages upon storage and differences in the relative abundance of each of its isomer occur when reconstituted in an aqueous solution. While all three of the dimeric forms of DHA ultimately resolve to the ketone and hydrated forms of monomeric DHA once in water at room temperature, these species require hours rather than minutes to reach an equilibrium favoring the monomeric species. Consequently, when used in bolus or flux experiments, the relative abundance of each isomer and its effects at the time of application is dependent on the initial DHA isomeric composition and concentration, and time of equilibration in solution before use. Here, we make recommendations for the more consistent handling of DHA as we report conditions that ensure that DHA is present in its monomeric form while in solutions, conditions used in an isotopic tracing study that specifically compared monomeric DHA and GA metabolism in cells.
Texto completo:
1
Base de dados:
MEDLINE
Assunto principal:
Di-Hidroxiacetona
/
Sistemas Eletrônicos de Liberação de Nicotina
Idioma:
En
Ano de publicação:
2022
Tipo de documento:
Article