A morphometric analysis of the osteocyte canaliculus using applied automatic semantic segmentation by machine learning.
J Bone Miner Metab
; 40(4): 571-580, 2022 Jul.
Article
em En
| MEDLINE
| ID: mdl-35338405
ABSTRACT
INTRODUCTION:
Osteocytes play a role as mechanosensory cells by sensing flow-induced mechanical stimuli applied on their cell processes. High-resolution imaging of osteocyte processes and the canalicular wall are necessary for the analysis of this mechanosensing mechanism. Focused ion beam-scanning electron microscopy (FIB-SEM) enabled the visualization of the structure at the nanometer scale with thousands of serial-section SEM images. We applied machine learning for the automatic semantic segmentation of osteocyte processes and canalicular wall and performed a morphometric analysis using three-dimensionally reconstructed images. MATERIALS ANDMETHODS:
Six-week-old-mice femur were used. Osteocyte processes and canaliculi were observed at a resolution of 2 nm/voxel in a 4 × 4 µm region with 2000 serial-section SEM images. Machine learning was used for automatic semantic segmentation of the osteocyte processes and canaliculi from serial-section SEM images. The results of semantic segmentation were evaluated using the dice similarity coefficient (DSC). The segmented data were reconstructed to create three-dimensional images and a morphological analysis was performed.RESULTS:
The DSC was > 83%. Using the segmented data, a three-dimensional image of approximately 3.5 µm in length was reconstructed. The morphometric analysis revealed that the median osteocyte process diameter was 73.8 ± 18.0 nm, and the median pericellular fluid space around the osteocyte process was 40.0 ± 17.5 nm.CONCLUSION:
We used machine learning for the semantic segmentation of osteocyte processes and canalicular wall for the first time, and performed a morphological analysis using three-dimensionally reconstructed images.Palavras-chave
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Base de dados:
MEDLINE
Assunto principal:
Osteócitos
/
Imageamento Tridimensional
/
Aprendizado de Máquina
Limite:
Animals
Idioma:
En
Ano de publicação:
2022
Tipo de documento:
Article