A novel isoform of hydroxyacyl-CoA dehydrogenase inhibits cell proliferation.
Biochem Biophys Res Commun
; 606: 75-79, 2022 05 28.
Article
em En
| MEDLINE
| ID: mdl-35339755
ABSTRACT
Hydroxyacyl-CoA dehydrogenase (HADH) catalyzes the third reaction of mitochondrial ß-oxidation cascade, while the regulation of its expression and function remains to be elucidated. Using the quantitative translation initiation sequencing (QTI-seq), we have identified that murine Hadh mRNA has two alternative translation start codons. We demonstrated that translation from upstream start codon encodes the mitochondrial isoform of HADH, while translation from downstream start codon produces a short isoform (HADH-S) with predominant nuclear localization. Moreover, overexpression of HADH-S inhibits the proliferation of mouse embryonic fibroblasts. Overall, our results identify a novel isoform of HADH participating in cell proliferation.
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Base de dados:
MEDLINE
Assunto principal:
Fibroblastos
/
3-Hidroxiacil-CoA Desidrogenases
Limite:
Animals
Idioma:
En
Ano de publicação:
2022
Tipo de documento:
Article