Human cytomegalovirus lytic infection inhibits replication-dependent histone synthesis and requires stem loop binding protein function.
Proc Natl Acad Sci U S A
; 119(14): e2122174119, 2022 04 05.
Article
em En
| MEDLINE
| ID: mdl-35344424
ABSTRACT
Replication-dependent (RD) histones are deposited onto human cytomegalovirus (HCMV) genomes at the start of infection. We examined how HCMV affects the de novo production of RD histones and found that viral infection blocked the accumulation of RD histone mRNAs that normally occurs during the S phase. Furthermore, RD histone mRNAs present in HCMV-infected cells did not undergo the unique 3' processing required for their normal nuclear export and translation. The protein that orchestrates processing in the nucleus, stem loopbinding protein (SLBP), was found predominantly in the cytoplasm, and RD histone proteins were not de novo synthesized in HCMV-infected cells. Intriguingly, however, we found that SLBP was required for the efficient synthesis and assembly of infectious progeny virions. We conclude that HCMV infection attenuates RD histone mRNA accumulation and processing and the de novo protein synthesis of the RD histones, while utilizing SLBP for an alternative purpose to support infectious virion production.
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Texto completo:
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Base de dados:
MEDLINE
Assunto principal:
Replicação Viral
/
Histonas
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Infecções por Citomegalovirus
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Citomegalovirus
Limite:
Humans
Idioma:
En
Ano de publicação:
2022
Tipo de documento:
Article