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RNA is required for the integrity of multiple nuclear and cytoplasmic membrane-less RNP granules.
Decker, Carolyn J; Burke, James M; Mulvaney, Patrick K; Parker, Roy.
Afiliação
  • Decker CJ; Department of Biochemistry, University of Colorado Boulder, Boulder, CO, USA.
  • Burke JM; Howard Hughes Medical Institute, University of Colorado Boulder, Boulder, CO, USA.
  • Mulvaney PK; Department of Biochemistry, University of Colorado Boulder, Boulder, CO, USA.
  • Parker R; Department of Biochemistry, University of Colorado Boulder, Boulder, CO, USA.
EMBO J ; 41(9): e110137, 2022 05 02.
Article em En | MEDLINE | ID: mdl-35355287
Numerous membrane-less organelles, composed of a combination of RNA and proteins, are observed in the nucleus and cytoplasm of eukaryotic cells. These RNP granules include stress granules (SGs), processing bodies (PBs), Cajal bodies, and nuclear speckles. An unresolved question is how frequently RNA molecules are required for the integrity of RNP granules in either the nucleus or cytosol. To address this issue, we degraded intracellular RNA in either the cytosol or the nucleus by the activation of RNase L and examined the impact of RNA loss on several RNP granules. We find the majority of RNP granules, including SGs, Cajal bodies, nuclear speckles, and the nucleolus, are altered by the degradation of their RNA components. In contrast, PBs and super-enhancer complexes were largely not affected by RNA degradation in their respective compartments. RNA degradation overall led to the apparent dissolution of some membrane-less organelles, whereas others reorganized into structures with altered morphology. These findings highlight a critical and widespread role of RNA in the organization of several RNP granules.
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Texto completo: 1 Base de dados: MEDLINE Assunto principal: RNA / Grânulos Citoplasmáticos Idioma: En Ano de publicação: 2022 Tipo de documento: Article

Texto completo: 1 Base de dados: MEDLINE Assunto principal: RNA / Grânulos Citoplasmáticos Idioma: En Ano de publicação: 2022 Tipo de documento: Article