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Correlations between DWI, IVIM, and HIF-1α expression based on MRI and pathology in a murine model of rhabdomyosarcoma.
Yin, Zhenzhen; Li, Xiangwen; Zhang, Yu; Tao, Juan; Yang, Yanyu; Fang, Shaobo; Zhang, Zhengyang; Yuan, Yuan; Liu, Yajie; Wang, Shaowu.
Afiliação
  • Yin Z; Department of Radiology, The Second Hospital, Dalian Medical University, Dalian, Liaoning, People's Republic of China.
  • Li X; Department of Radiology, Suzhou Hospital of Anhui Medical University, Suzhou, Anhui, People's Republic of China.
  • Zhang Y; Department of Radiology, Huashan Hospital affiliated to Fudan University, Shanghai, People's Republic of China.
  • Tao J; Department of Radiology, The Second Hospital, Dalian Medical University, Dalian, Liaoning, People's Republic of China.
  • Yang Y; Department of Pathology, The Second Hospital, Dalian Medical University, Dalian, Liaoning, People's Republic of China.
  • Fang S; Department of Radiology, The Second Hospital, Dalian Medical University, Dalian, Liaoning, People's Republic of China.
  • Zhang Z; Department of Radiology, The Second Hospital, Dalian Medical University, Dalian, Liaoning, People's Republic of China.
  • Yuan Y; Department of Radiology, The Second Hospital, Dalian Medical University, Dalian, Liaoning, People's Republic of China.
  • Liu Y; Department of Radiology, The Second Hospital, Dalian Medical University, Dalian, Liaoning, People's Republic of China.
  • Wang S; Department of Radiology, The Second Hospital, Dalian Medical University, Dalian, Liaoning, People's Republic of China.
Magn Reson Med ; 88(2): 871-879, 2022 08.
Article em En | MEDLINE | ID: mdl-35377480
PURPOSE: To investigate the correlation between DWI, intravoxel incoherent motion (IVIM), and hypoxia-inducible factor 1-alpha (HIF-1α) expression in a nude mouse model of rhabdomyosarcoma based on imaging and pathological comparisons. METHODS: Human rhabdomyosarcoma-derived (RD) cells were inoculated into the right thigh muscle of 20 BALB/c female nude mice. Mice were imaged using 3.0 Tesla MRI system. T1 -weighted imaging, T2 -weighted imaging, DWI, and IVIM images were obtained. ADW4.7 (GE Healthcare, ChicagoAQ34, IL, USA) was used for image processing of ADC, Dslow , Dfast , and f values. All parameter values were independently analyzed by 2 observers. Immunohistochemistry of HIF-1α was performed. We used a specific image-pathology comparison method to ensure correct overlap between the image plane and the pathological section. Mann-Whitney U test or independent sample t test, Pearson or Spearman correlation test, the intragroup correlation coefficient, Kolmogorov-Smirnov test, and receiver operating characteristic curve were used. The correlation between DWI and intravoxel incoherent motion parameter values and HIF-1α expression was determined. RESULTS: There were 10 mice in the low-expression group and 7 in the high-expression group. The ADC and Dslow values were negatively correlated with HIF-1α with correlation coefficients of -0.491 and - 0.702 (P = 0.045 and 0.002). The f value positively correlated with HIF-1α expression (r = 0.485, P = 0.048). ADC, Dslow , and f were significantly different between the high-HIF-1α expression tumors and the low-HIF-1α expression tumors. ADC showed the best predictive performance among all parameters (area under the curve = 0.652, sensitivity = 83.3%, specificity = 63.6%). CONCLUSION: The parameter values of DWI and intravoxel incoherent motion can be used to evaluate the expression of HIF-1α in rhabdomyosarcoma. ADC, Dslow , and f value showed correlation with the expression of HIF-1α.
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Texto completo: 1 Base de dados: MEDLINE Assunto principal: Rabdomiossarcoma / Imagem de Difusão por Ressonância Magnética Tipo de estudo: Prognostic_studies Limite: Animals Idioma: En Ano de publicação: 2022 Tipo de documento: Article

Texto completo: 1 Base de dados: MEDLINE Assunto principal: Rabdomiossarcoma / Imagem de Difusão por Ressonância Magnética Tipo de estudo: Prognostic_studies Limite: Animals Idioma: En Ano de publicação: 2022 Tipo de documento: Article