Single-cell transcriptional diversity of neonatal umbilical cord blood immune cells reveals neonatal immune tolerance.
Biochem Biophys Res Commun
; 608: 14-22, 2022 06 11.
Article
em En
| MEDLINE
| ID: mdl-35381424
ABSTRACT
The characteristics of neonatal immune cells display intrinsic differences compared with adult immune cells. Therefore, a comprehensive analysis of key gene expression regulation is required to understand the response of the human fetal immune system to infections. Here, we applied single-cell RNA sequencing (scRNA-seq) and single-cell sequencing assay for transposase-accessible chromatin (scATAC-seq) to systematically profile umbilical cord blood (UCB) nucleated cells and peripheral blood mononuclear cells (PBMCs) to identify their composition and differentially expressed genes. The immune cells in neonatal UCB demonstrated the expression of key genes, such as HBG2, NFKBIA, JUN, FOS, and TNFAIP3. In contrast, natural killer and T cells, which are constituents of adult PBMCs, exhibited high cytotoxic gene expression. Furthermore, we obtained similar results from the data of scATAC-seq by identifying the status of chromatin accessibility of key genes. Therefore, scRNA-seq and scATAC-seq of neonatal UCB nucleated cells and adult PBMCs could serve as an invaluable resource for elucidating the regulatory mechanisms of responses of distinct immune cell types and further identifying the differences between neonatal and adult immune responses to predict the potential underlying mechanism for neonatal immune tolerance.
Palavras-chave
Texto completo:
1
Base de dados:
MEDLINE
Assunto principal:
Sangue Fetal
/
Análise de Célula Única
Tipo de estudo:
Prognostic_studies
Limite:
Adult
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Humans
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Newborn
Idioma:
En
Ano de publicação:
2022
Tipo de documento:
Article