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Mad2 promotes Cyclin B2 recruitment to the kinetochore for guiding accurate mitotic checkpoint.
Liu, Sikai; Yuan, Xiao; Gui, Ping; Liu, Ran; Durojaye, Olanrewaju; Hill, Donald L; Fu, Chuanhai; Yao, Xuebiao; Dou, Zhen; Liu, Xing.
Afiliação
  • Liu S; MOE Key Laboratory for Cellular Dynamics and The First Affiliated Hospital, School of Life Sciences, University of Science and Technology of China, Hefei, China.
  • Yuan X; CAS Center for Excellence in Molecular Cell Science, Chinese Academy of Sciences, Shanghai, China.
  • Gui P; MOE Key Laboratory for Cellular Dynamics and The First Affiliated Hospital, School of Life Sciences, University of Science and Technology of China, Hefei, China.
  • Liu R; CAS Center for Excellence in Molecular Cell Science, Chinese Academy of Sciences, Shanghai, China.
  • Durojaye O; MOE Key Laboratory for Cellular Dynamics and The First Affiliated Hospital, School of Life Sciences, University of Science and Technology of China, Hefei, China.
  • Hill DL; CAS Center for Excellence in Molecular Cell Science, Chinese Academy of Sciences, Shanghai, China.
  • Fu C; MOE Key Laboratory for Cellular Dynamics and The First Affiliated Hospital, School of Life Sciences, University of Science and Technology of China, Hefei, China.
  • Yao X; CAS Center for Excellence in Molecular Cell Science, Chinese Academy of Sciences, Shanghai, China.
  • Dou Z; MOE Key Laboratory for Cellular Dynamics and The First Affiliated Hospital, School of Life Sciences, University of Science and Technology of China, Hefei, China.
  • Liu X; CAS Center for Excellence in Molecular Cell Science, Chinese Academy of Sciences, Shanghai, China.
EMBO Rep ; 23(6): e54171, 2022 06 07.
Article em En | MEDLINE | ID: mdl-35384228
Accurate mitotic progression relies on the dynamic phosphorylation of multiple substrates by key mitotic kinases. Cyclin-dependent kinase 1 is a master kinase that coordinates mitotic progression and requires its regulatory subunit Cyclin B to ensure full kinase activity and substrate specificity. The function of Cyclin B2, which is a closely related family member of Cyclin B1, remains largely elusive. Here, we show that Mad2 promotes the kinetochore localization of Cyclin B2 and that their interaction at the kinetochores guides accurate chromosome segregation. Our biochemical analyses have characterized the Mad2-Cyclin B2 interaction and delineated a novel Mad2-interacting motif (MIM) on Cyclin B2. The functional importance of the Cyclin B2-Mad2 interaction was demonstrated by real-time imaging in which MIM-deficient mutant Cyclin B2 failed to rescue the chromosomal segregation defects. Taken together, we have delineated a previously undefined function of Cyclin B2 at the kinetochore and have established, in human cells, a mechanism of action by which Mad2 contributes to the spindle checkpoint.
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Texto completo: 1 Base de dados: MEDLINE Assunto principal: Cinetocoros / Ciclina B2 / Pontos de Checagem da Fase M do Ciclo Celular / Proteínas Mad2 Limite: Humans Idioma: En Ano de publicação: 2022 Tipo de documento: Article

Texto completo: 1 Base de dados: MEDLINE Assunto principal: Cinetocoros / Ciclina B2 / Pontos de Checagem da Fase M do Ciclo Celular / Proteínas Mad2 Limite: Humans Idioma: En Ano de publicação: 2022 Tipo de documento: Article