Analyses of murine lymph node endothelial cell subsets using single-cell RNA sequencing and spectral flow cytometry.
STAR Protoc
; 3(2): 101267, 2022 06 17.
Article
em En
| MEDLINE
| ID: mdl-35403006
ABSTRACT
Blood endothelial cells (BECs) in lymph nodes are distinct stromal cells with a transcriptional profile allowing fast and specific adaptation to the functional requirements. Here, we describe a step-by-step protocol for the enzymatic digestion of lymph nodes, the enrichment of stromal cells, the sorting of BECs, and the processing of BEC-related data for modern analysis approaches as spectral flow cytometry and single-cell RNA sequencing (scRNA-seq). For complete details on the use and execution of this protocol, please refer to Menzel et al. (2021).
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Texto completo:
1
Base de dados:
MEDLINE
Assunto principal:
Células Endoteliais
/
Análise de Célula Única
Limite:
Animals
Idioma:
En
Ano de publicação:
2022
Tipo de documento:
Article