Control of Nucleophile Chemoselectivity in Cyanobactin YcaO Heterocyclases PatD and TruD.
ACS Chem Biol
; 17(5): 1215-1225, 2022 05 20.
Article
em En
| MEDLINE
| ID: mdl-35420020
ABSTRACT
Members of the YcaO superfamily are among the most common post-translational modification enzymes in natural product biosynthesis, with wide usage in biotechnology and synthetic biology applications. Here, we use domain-swapped chimeras and discovered unstructured regions in cyanobactin YcaOs that guide interactions with the substrates, governing access to interior amino acids in the substrates and explaining the chemoselectivity between PatD and TruD. These results define how the cyanobactin heterocyclases modify exceptionally sequence diverse substrates, yet with a high degree of positional and nucleophile selectivity.
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1
Base de dados:
MEDLINE
Assunto principal:
Peptídeos Cíclicos
/
Processamento de Proteína Pós-Traducional
Idioma:
En
Ano de publicação:
2022
Tipo de documento:
Article