A single-cell liver atlas of Plasmodium vivax infection.
Cell Host Microbe
; 30(7): 1048-1060.e5, 2022 07 13.
Article
em En
| MEDLINE
| ID: mdl-35443155
Malaria-causing Plasmodium vivax parasites can linger in the human liver for weeks to years and reactivate to cause recurrent blood-stage infection. Although they are an important target for malaria eradication, little is known about the molecular features of replicative and non-replicative intracellular liver-stage parasites and their host cell dependence. Here, we leverage a bioengineered human microliver platform to culture patient-derived P. vivax parasites for transcriptional profiling. Coupling enrichment strategies with bulk and single-cell analyses, we capture both parasite and host transcripts in individual hepatocytes throughout the course of infection. We define host- and state-dependent transcriptional signatures and identify unappreciated populations of replicative and non-replicative parasites that share features with sexual transmissive forms. We find that infection suppresses the transcription of key hepatocyte function genes and elicits an anti-parasite innate immune response. Our work provides a foundation for understanding host-parasite interactions and reveals insights into the biology of P. vivax dormancy and transmission.
Palavras-chave
Texto completo:
1
Base de dados:
MEDLINE
Assunto principal:
Malária Vivax
/
Malária
Limite:
Humans
Idioma:
En
Ano de publicação:
2022
Tipo de documento:
Article