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Combined protein and nucleic acid imaging reveals virus-dependent B cell and macrophage immunosuppression of tissue microenvironments.
Jiang, Sizun; Chan, Chi Ngai; Rovira-Clavé, Xavier; Chen, Han; Bai, Yunhao; Zhu, Bokai; McCaffrey, Erin; Greenwald, Noah F; Liu, Candace; Barlow, Graham L; Weirather, Jason L; Oliveria, John Paul; Nakayama, Tsuguhisa; Lee, Ivan T; Matter, Matthias S; Carlisle, Anne E; Philips, Darci; Vazquez, Gustavo; Mukherjee, Nilanjan; Busman-Sahay, Kathleen; Nekorchuk, Michael; Terry, Margaret; Younger, Skyler; Bosse, Marc; Demeter, Janos; Rodig, Scott J; Tzankov, Alexandar; Goltsev, Yury; McIlwain, David Robert; Angelo, Michael; Estes, Jacob D; Nolan, Garry P.
Afiliação
  • Jiang S; Department of Pathology, Stanford University, Stanford, CA, USA; Center for Virology and Vaccine Research, Beth Israel Deaconess Medical Center, Harvard Medical School, Boston, MA, USA. Electronic address: sjiang3@bidmc.harvard.edu.
  • Chan CN; Vaccine and Gene Therapy Institute, Oregon Health & Science University, Beaverton, OR, USA.
  • Rovira-Clavé X; Department of Pathology, Stanford University, Stanford, CA, USA.
  • Chen H; Department of Pathology, Stanford University, Stanford, CA, USA.
  • Bai Y; Department of Pathology, Stanford University, Stanford, CA, USA.
  • Zhu B; Department of Pathology, Stanford University, Stanford, CA, USA.
  • McCaffrey E; Department of Pathology, Stanford University, Stanford, CA, USA.
  • Greenwald NF; Department of Pathology, Stanford University, Stanford, CA, USA.
  • Liu C; Department of Pathology, Stanford University, Stanford, CA, USA.
  • Barlow GL; Department of Pathology, Stanford University, Stanford, CA, USA.
  • Weirather JL; Center of Immuno-Oncology, Dana-Faber Cancer Institute, Boston, MA, USA.
  • Oliveria JP; Department of Pathology, Stanford University, Stanford, CA, USA; Department of Medicine, McMaster University, Hamilton, ON, Canada.
  • Nakayama T; Department of Pathology, Stanford University, Stanford, CA, USA; Department of Otorhinolaryngology, Jikei University School of Medicine, Tokyo, Japan.
  • Lee IT; Department of Pathology, Stanford University, Stanford, CA, USA; Division of Allergy, Immunology, and Rheumatology, Department of Pediatrics, Stanford University School of Medicine, Stanford, CA, USA.
  • Matter MS; Pathology, Institute of Medical Genetics and Pathology, University Hospital Basel, University of Basel, Basel, Switzerland.
  • Carlisle AE; Center of Immuno-Oncology, Dana-Faber Cancer Institute, Boston, MA, USA.
  • Philips D; Department of Pathology, Stanford University, Stanford, CA, USA.
  • Vazquez G; Department of Pathology, Stanford University, Stanford, CA, USA.
  • Mukherjee N; Department of Pathology, Stanford University, Stanford, CA, USA.
  • Busman-Sahay K; Vaccine and Gene Therapy Institute, Oregon Health & Science University, Beaverton, OR, USA.
  • Nekorchuk M; Vaccine and Gene Therapy Institute, Oregon Health & Science University, Beaverton, OR, USA.
  • Terry M; Vaccine and Gene Therapy Institute, Oregon Health & Science University, Beaverton, OR, USA.
  • Younger S; Vaccine and Gene Therapy Institute, Oregon Health & Science University, Beaverton, OR, USA.
  • Bosse M; Department of Pathology, Stanford University, Stanford, CA, USA.
  • Demeter J; Department of Pathology, Stanford University, Stanford, CA, USA.
  • Rodig SJ; Department of Pathology, Brigham & Women's Hospital, Boston, MA, USA.
  • Tzankov A; Pathology, Institute of Medical Genetics and Pathology, University Hospital Basel, University of Basel, Basel, Switzerland.
  • Goltsev Y; Department of Pathology, Stanford University, Stanford, CA, USA.
  • McIlwain DR; Department of Pathology, Stanford University, Stanford, CA, USA.
  • Angelo M; Department of Pathology, Stanford University, Stanford, CA, USA.
  • Estes JD; Vaccine and Gene Therapy Institute, Oregon Health & Science University, Beaverton, OR, USA; Division of Pathobiology & Immunology, Oregon National Primate Research Center, Oregon Health & Science University, Beaverton, OR, USA. Electronic address: estesja@ohsu.edu.
  • Nolan GP; Department of Pathology, Stanford University, Stanford, CA, USA. Electronic address: gnolan@stanford.edu.
Immunity ; 55(6): 1118-1134.e8, 2022 06 14.
Article em En | MEDLINE | ID: mdl-35447093
ABSTRACT
Understanding the mechanisms of HIV tissue persistence necessitates the ability to visualize tissue microenvironments where infected cells reside; however, technological barriers limit our ability to dissect the cellular components of these HIV reservoirs. Here, we developed protein and nucleic acid in situ imaging (PANINI) to simultaneously quantify DNA, RNA, and protein levels within these tissue compartments. By coupling PANINI with multiplexed ion beam imaging (MIBI), we measured over 30 parameters simultaneously across archival lymphoid tissues from healthy or simian immunodeficiency virus (SIV)-infected nonhuman primates. PANINI enabled the spatial dissection of cellular phenotypes, functional markers, and viral events resulting from infection. SIV infection induced IL-10 expression in lymphoid B cells, which correlated with local macrophage M2 polarization. This highlights a potential viral mechanism for conditioning an immunosuppressive tissue environment for virion production. The spatial multimodal framework here can be extended to decipher tissue responses in other infectious diseases and tumor biology.
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Texto completo: 1 Base de dados: MEDLINE Assunto principal: Ácidos Nucleicos / Infecções por HIV / Síndrome de Imunodeficiência Adquirida dos Símios / Vírus da Imunodeficiência Símia Limite: Animals Idioma: En Ano de publicação: 2022 Tipo de documento: Article
Texto completo
Imprimir
XML
PubMed Links
Buscar no Google

Texto completo: 1 Base de dados: MEDLINE Assunto principal: Ácidos Nucleicos / Infecções por HIV / Síndrome de Imunodeficiência Adquirida dos Símios / Vírus da Imunodeficiência Símia Limite: Animals Idioma: En Ano de publicação: 2022 Tipo de documento: Article