Comparison of special stains (Giemsa stain and Modified Toluidine Blue stain) with immunohistochemistry as gold standard for the detection of H. pylori in gastric biopsies.
Arab J Gastroenterol
; 23(2): 75-81, 2022 May.
Article
em En
| MEDLINE
| ID: mdl-35484045
ABSTRACT
BACKGROUND AND STUDY AIMS:
Helicobacter pylori (H. pylori) has been extensively implicated in the etiology and pathogenesis of gastric ulcers and carcinomas, which has necessitated its efficient and cost-effective identification in gastric biopsy samples. We have sought to compare various staining methods, namely, Hematoxylin and Eosin (H&E), Giemsa, and Modified Toluidine Blue (MTB), with immunohistochemistry (IHC; gold standard) in terms of efficacy, staining costs, and duration of performing the stains in settings with limited resources. PATIENTS ANDMETHODS:
Gastric biopsy specimens of 50 patients who presented with gastritis symptoms were stained with H&E, Giemsa, MTB, and IHC. The sensitivity, specificity, positive/negative predictive values, and the receiver operating characteristic curve were calculated for each stain.RESULTS:
In all, 32 cases of 50 were positive for H. pylori on IHC. The specificity for both H&E and Giemsa was 88.8%, whereas it was lower for MTB (83.3%). The sensitivity of H&E was much lower (46.8%) compared with the other stains (90.6% Giemsa, 93.7% MTB). The most inexpensive and time-consuming stain was H&E followed by MTB and Giemsa.CONCLUSION:
When H&E is used alone for H. pylori detection, a significant number of cases may be overlooked, especially mild inflammatory cases and when coccoid forms of the bacteria are present. This study proposes the use of either Giemsa or MTB as reliable alternatives for IHC in resource-limited settings to combat the high prevalence of H. pylori in the developing world.Palavras-chave
Texto completo:
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Base de dados:
MEDLINE
Assunto principal:
Helicobacter pylori
/
Infecções por Helicobacter
Tipo de estudo:
Diagnostic_studies
/
Prognostic_studies
/
Risk_factors_studies
Limite:
Humans
Idioma:
En
Ano de publicação:
2022
Tipo de documento:
Article