3D Immunofluorescent Image Colocalization Quantification in Mouse Epiblast Stem Cells.

Dierolf, Joshua G; Watson, Andrew J; Betts, Dean H

Dierolf, Joshua G; Watson, Andrew J; Betts, Dean H.

Afiliação

Dierolf JG; Department of Physiology and Pharmacology, Schulich School of Medicine & Dentistry, The University of Western Ontario, London, ON, Canada.
Watson AJ; Department of Physiology and Pharmacology, Schulich School of Medicine & Dentistry, The University of Western Ontario, London, ON, Canada.
Betts DH; Department of Obstetrics and Gynecology, Schulich School of Medicine & Dentistry, The University of Western Ontario, London, ON, Canada.

Methods Mol Biol ; 2490: 69-79, 2022.

Article em En | MEDLINE | ID: mdl-35486240

ABSTRACT

ABSTRACT

This chapter details 3D morphological topography of colony architecture optimization and nuclear protein localization by co-immunofluorescent confocal microscopy analysis. Colocalization assessment of nuclear and cytoplasmic cell regions is detailed to demonstrate nuclear and cytoplasmic localization in mEpiSCs by confocal microscopy and orthogonal colocalization assessment. Protein colocalization within mESCs, mEpiLCs, and mEpiSCs can be efficiently completed using these optimized protocols.

Assuntos

Camadas Germinativas; Células-Tronco Embrionárias Murinas; Animais; Núcleo Celular; Corantes; Citoplasma; Camundongos; Microscopia Confocal

Palavras-chave

Colocalization; Confocal microscopy; Cytoplasmic; Immunofluorescence; Nuclear; Primed pluripotency; mEpiSC

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Texto completo: 1 Base de dados: MEDLINE Assunto principal: Células-Tronco Embrionárias Murinas / Camadas Germinativas Limite: Animals Idioma: En Ano de publicação: 2022 Tipo de documento: Article

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