More than one way to bind to cholesterol: atypical variants of membrane-binding domain of perfringolysin O selected by ribosome display.
RSC Adv
; 10(63): 38678-38682, 2020 Oct 15.
Article
em En
| MEDLINE
| ID: mdl-35517550
ABSTRACT
Herein, we report a high-throughput approach for the selection of peripheral protein domains that bind specifically to cholesterol in lipid membranes. We discovered variants of perfringolysin O, with non-conserved amino acid substitutions at regions crucial for cholesterol recognition, demonstrating an unprecedented amino acid sequence variability with binding ability for cholesterol. The developed approach provides an effective platform for a comprehensive study of protein lipid interactions.
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MEDLINE
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En
Ano de publicação:
2020
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Article