Your browser doesn't support javascript.
loading
Patient-derived bladder cancer xenograft models reveal VEGF and CDK4 enhancing tumor metastasis behavior.
Zhao, Yong; An, Mingjie; Zhang, He; Tan, Dengxu; Chen, Xue; Wu, Pengpeng; Qin, Weijun; Zhang, Caiqin; Shi, Changhong.
Afiliação
  • Zhao Y; Division of Cancer Biology, Laboratory Animal Center, The Fourth Military Medical University Xi'an Shaanxi 710032 China changhong@fmmu.edu.cn zhangcaiqin-beibei@163.com.
  • An M; MBBS Zhongshan School of Medicine, Sun Yat-sen University Guangzhou 510080 China.
  • Zhang H; Division of Cancer Biology, Laboratory Animal Center, The Fourth Military Medical University Xi'an Shaanxi 710032 China changhong@fmmu.edu.cn zhangcaiqin-beibei@163.com.
  • Tan D; Division of Cancer Biology, Laboratory Animal Center, The Fourth Military Medical University Xi'an Shaanxi 710032 China changhong@fmmu.edu.cn zhangcaiqin-beibei@163.com.
  • Chen X; Division of Cancer Biology, Laboratory Animal Center, The Fourth Military Medical University Xi'an Shaanxi 710032 China changhong@fmmu.edu.cn zhangcaiqin-beibei@163.com.
  • Wu P; Division of Cancer Biology, Laboratory Animal Center, The Fourth Military Medical University Xi'an Shaanxi 710032 China changhong@fmmu.edu.cn zhangcaiqin-beibei@163.com.
  • Qin W; Department of Urology, Xijing Hospital, The Fourth Military Medical University Xi'an Shaanxi 710032 China.
  • Zhang C; Division of Cancer Biology, Laboratory Animal Center, The Fourth Military Medical University Xi'an Shaanxi 710032 China changhong@fmmu.edu.cn zhangcaiqin-beibei@163.com.
  • Shi C; Division of Cancer Biology, Laboratory Animal Center, The Fourth Military Medical University Xi'an Shaanxi 710032 China changhong@fmmu.edu.cn zhangcaiqin-beibei@163.com.
RSC Adv ; 9(31): 17877-17884, 2019 Jun 04.
Article em En | MEDLINE | ID: mdl-35520551
New strategies to treat advanced bladder cancer are urgently required. A patient-derived xenograft (PDX) model has become a useful tool to evaluate chemotherapeutics and investigate personalized cancer treatment options. In this study, fresh human bladder cancer specimens (C09303 and C21391) were transplanted subcutaneously into nude mice to establish PDX models. These models well retained pathological characteristics and molecular markers of the original tumor. Primary cells derived from C09303 were cultured and perfused into the bladders of nude mice to establish orthotopic xenograft models. Evident signals of lung and kidney metastases were detected by whole-body optical imaging. Gemcitabine displayed a significant therapeutic effect on the subcutaneous or orthotopic xenograft tumors of C09303. In vitro, matrigel invasion assays showed that C09303 primary culture cells are remarkably invasive. To study the metastatic properties of C09303, we sequenced the genomes of C09303 and C21391, and found that the expression of VEGF and CDK4 was significantly upregulated, and VEGF-knockdown or CDK4-knockdown C09303 cells showed attenuated invasiveness and migration. This PDX model revealed that VEGF and CDK4 enhanced tumor metastasis behavior, suggesting them as novel molecular therapeutic targets. This framework provides a tool for research on metastasis mechanism and individualized treatment for bladder cancer.

Texto completo: 1 Base de dados: MEDLINE Tipo de estudo: Prognostic_studies Idioma: En Ano de publicação: 2019 Tipo de documento: Article

Texto completo: 1 Base de dados: MEDLINE Tipo de estudo: Prognostic_studies Idioma: En Ano de publicação: 2019 Tipo de documento: Article