Label-free identification carbapenem-resistant Escherichia coli based on surface-enhanced resonance Raman scattering.

ABSTRACT

In this study, a surface-enhanced resonance Raman scattering (SERRS) method has been developed for the accurate detection and identification of carbapenem-resistant and carbapenem-sensitive Escherichia coli. A total of 89 human isolates of Enterobacteriaceae, comprising 41 strains of carbapenem-sensitive E. coli (CSEC) and 48 strains of carbapenem-resistant E. coli (CREC), were tested to assess the feasibility of our proposed SERRS method as a clinical tool, and the results showed almost 100% accuracy.