A novel alveolar epithelial cell sheet fabricated under feeder-free conditions for potential use in pulmonary regenerative therapy.

Mitsuboshi, Shota; Homma, Jun; Sekine, Hidekazu; Takagi, Ryo; Shimizu, Tatsuya; Kanzaki, Masato.

Afiliação

Mitsuboshi S; Department of Thoracic Surgery, Tokyo Women's Medical University, 8-1 Kawada-cho, Shinjuku-ku, Tokyo 162-8666, Japan.
Homma J; Institute of Advanced Biomedical Engineering and Science, Tokyo Women's Medical University, 8-1 Kawada-cho, Shinjuku-ku, Tokyo 162-8666, Japan.
Sekine H; Institute of Advanced Biomedical Engineering and Science, Tokyo Women's Medical University, 8-1 Kawada-cho, Shinjuku-ku, Tokyo 162-8666, Japan.
Takagi R; Institute of Advanced Biomedical Engineering and Science, Tokyo Women's Medical University, 8-1 Kawada-cho, Shinjuku-ku, Tokyo 162-8666, Japan.
Shimizu T; Institute of Advanced Biomedical Engineering and Science, Tokyo Women's Medical University, 8-1 Kawada-cho, Shinjuku-ku, Tokyo 162-8666, Japan.
Kanzaki M; Department of Thoracic Surgery, Tokyo Women's Medical University, 8-1 Kawada-cho, Shinjuku-ku, Tokyo 162-8666, Japan.

Regen Ther ; 19: 113-121, 2022 Mar.

Article em En | MEDLINE | ID: mdl-35582208

Palavras-chave

AEC, alveolar epithelial cell; AECI, type I alveolar epithelial cell; AECII, type II alveolar epithelial cell; AEpiCM, alveolar epithelial cell medium; AQP-5, aquaporin-5; Alveolar epithelial cell; Ca2+, ionized calcium; Cell sheet; FBS, fetal bovine serum; Feeder-free; GFP, green fluorescent protein; HBSS, Hanks' balanced salt solution; HE, hematoxylin and eosin; LCM, medium with a low ionized calcium concentration; PBS, phosphate-buffered saline; ROCK, rho kinase; Regenerative therapy; SP, surfactant protein; rLN511E8, recombinantly expressed laminin-511 E8 fragment

Texto completo: 1 Base de dados: MEDLINE Idioma: En Ano de publicação: 2022 Tipo de documento: Article

Texto completo: 1 Base de dados: MEDLINE Idioma: En Ano de publicação: 2022 Tipo de documento: Article