Gap-enhance Raman tags (GERTs) competitive immunoassay based Raman imaging for the quantitative detection of trace florfenicol in milk.
Food Chem
; 391: 133233, 2022 Oct 15.
Article
em En
| MEDLINE
| ID: mdl-35605538
ABSTRACT
Surface Enhanced Raman Spectroscopy (SERS) is a highly sensitive detection method, however, it is still challenging to directly detect florfenicol due to its low Raman activity. To solve this problem, we present a novel sensor for selective detection of florfenicol residue in milk using gap-enhance Raman tags (GERTs) competitive immunoassay. In this nanosensor, Raman reporters 4-mercaptobenzoic acid (4MBA) were embedded in the 1 nm gap of Au core-gap-Au shell nanoparticles, which can protect 4MBA molecules from unwanted external environments and provide a high enhancement. The parameters affecting the detection sensitivity were discussed and optimized. The limit detection of florfenicol in milk can reach 0.1 µg/kg. To evaluate the performance of GERTs immunoassay, Ag@4MBA tags immunoassay was conducted and the results were compared. Moreover, Raman mapping was investigated to improve the quantitative prediction model with R2 of 0.981 achieved, by eliminating the signal fluctuations caused by the inhomogeneous distribution of GERTs.
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Texto completo:
1
Base de dados:
MEDLINE
Assunto principal:
Nanopartículas Metálicas
/
Ouro
Tipo de estudo:
Diagnostic_studies
/
Prognostic_studies
Limite:
Animals
Idioma:
En
Ano de publicação:
2022
Tipo de documento:
Article