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An Arabidopsis Retention and Splicing complex regulates root and embryo development through pre-mRNA splicing.
Xiong, Feng; Ren, Jing-Jing; Wang, Yu-Yi; Zhou, Zhou; Qi, Hao-Dong; Otegui, Marisa S; Wang, Xiu-Ling.
Afiliação
  • Xiong F; National Key Laboratory of Crop Biology, College of Life Sciences, Shandong Agricultural University, Taian 271018, China.
  • Ren JJ; National Key Laboratory of Crop Biology, College of Life Sciences, Shandong Agricultural University, Taian 271018, China.
  • Wang YY; National Key Laboratory of Crop Biology, College of Life Sciences, Shandong Agricultural University, Taian 271018, China.
  • Zhou Z; National Key Laboratory of Crop Biology, College of Life Sciences, Shandong Agricultural University, Taian 271018, China.
  • Qi HD; National Key Laboratory of Crop Biology, College of Life Sciences, Shandong Agricultural University, Taian 271018, China.
  • Otegui MS; Department of Botany, University of Wisconsin-Madison, Madison, Wisconsin 53706, USA.
  • Wang XL; Center for Quantitative Cell Imaging, University of Wisconsin-Madison, Madison, Wisconsin 53706, USA.
Plant Physiol ; 190(1): 621-639, 2022 08 29.
Article em En | MEDLINE | ID: mdl-35640107
ABSTRACT
Pre-mRNA splicing is an important step in the posttranscriptional processing of transcripts and a key regulator of development. The heterotrimeric retention and splicing (RES) complex plays vital roles in the growth and development of yeast, zebrafish, and humans by mediating pre-mRNA splicing of multiple genes. However, whether the RES complex is conserved in plants and what specific functions it has remain unknown. In this study, we identified Arabidopsis (Arabidopsis thaliana) BUD13 (AtBUD13), GROWTH, DEVELOPMENT AND SPLICING 1 (GDS1), and DAWDLE (DDL) as the counterparts of the yeast RES complex subunits Bud site selection protein 13 (Bud13), U2 snRNP component Snu17 (Snu17), and Pre-mRNA leakage protein 1, respectively. Moreover, we showed that RES is an ancient complex evolutionarily conserved in eukaryotes. GDS1 directly interacts with both AtBUD13 and DDL in nuclear speckles. The BUD13 domain of AtBUD13 and the RNA recognition motif domain of GDS1 are necessary and sufficient for AtBUD13-GDS1 interaction. Mutants of AtBUD13, GDS1, and DDL failed to properly splice multiple genes involved in cell proliferation and showed defects in early embryogenesis and root development. In addition, we found that GDS1 and DDL interact, respectively, with the U2 small nuclear ribonucleoproteins auxiliary factor AtU2AF65B and the NineTeen Complex-related splicing factor SKIP, which are essential for early steps of spliceosome assembly and recognition of splice sites. Altogether, our work reveals that the Arabidopsis RES complex is important for root and early embryo development by modulating pre-mRNA splicing.
Assuntos

Texto completo: 1 Base de dados: MEDLINE Assunto principal: Arabidopsis Tipo de estudo: Prognostic_studies Limite: Animals / Humans Idioma: En Ano de publicação: 2022 Tipo de documento: Article

Texto completo: 1 Base de dados: MEDLINE Assunto principal: Arabidopsis Tipo de estudo: Prognostic_studies Limite: Animals / Humans Idioma: En Ano de publicação: 2022 Tipo de documento: Article