Partial Tendon Injury at the Tendon-to-Bone Enthesis Activates Skeletal Stem Cells.
Stem Cells Transl Med
; 11(7): 715-726, 2022 07 20.
Article
em En
| MEDLINE
| ID: mdl-35640155
ABSTRACT
The tendon enthesis plays a critical role in facilitating movement and reducing stress within joints. Partial enthesis injuries heal in a mechanically inferior manner and never achieve healthy tissue function. The cells responsible for tendon-to-bone healing remain incompletely characterized and their origin is unknown. Here, we evaluated the putative role of mouse skeletal stem cells (mSSCs) in the enthesis after partial-injury. We found that mSSCs were present at elevated levels within the enthesis following injury and that these cells downregulated TGFß signaling pathway elements at both the RNA and protein levels. Exogenous application of TGFß post-injury led to a reduced mSSC response and impaired healing, whereas treatment with a TGFß inhibitor (SB43154) resulted in a more robust mSSC response. Collectively, these data suggest that mSSCs may augment tendon-to-bone healing by dampening the effects of TGFß signaling within the mSSC niche.
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Texto completo:
1
Base de dados:
MEDLINE
Assunto principal:
Traumatismos dos Tendões
/
Tendões
Limite:
Animals
Idioma:
En
Ano de publicação:
2022
Tipo de documento:
Article