Quality control of estrogen and progesterone receptor assays.
Ann Acad Med Singap
; 15(4): 492-7, 1986 Oct.
Article
em En
| MEDLINE
| ID: mdl-3566169
ABSTRACT
The intra-laboratory QC materials for estrogen and progesterone receptor assays were prepared using different methods. The most convenient and economical method was found to be the use of lyophilized rat uterine cytosol. The level of receptors remained stable for 12 months when stored at -70 degrees C with the inclusion of 10 mM phenylmethyl sulfonyl-fluoride in the cytosol extraction buffer. For inter-laboratory comparison, we received QC materials from Dr E D Ryan (McMaster University, Canada) monthly and our results fell within +/- 1SD of the mean value of 15 centres from North America and Europe participating in this program.
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Base de dados:
MEDLINE
Assunto principal:
Neoplasias da Mama
/
Receptores de Progesterona
/
Receptores de Estrogênio
Limite:
Animals
/
Female
/
Humans
Idioma:
En
Ano de publicação:
1986
Tipo de documento:
Article