Peptide supplementation relieves stress and enhances glycolytic flux in filamentous fungi during organic acid bioproduction.
Biotechnol Bioeng
; 119(9): 2471-2481, 2022 09.
Article
em En
| MEDLINE
| ID: mdl-35665482
ABSTRACT
Filamentous fungi occupy a uniquely favorable position in the bioproduction of organic acids. Intracellular stress is the main stimulator in filamentous fungi to produce and accumulate organic acids with high flux. However, stress can affect the physiological activities of filamentous fungi, thereby deteriorating their fermentation performance. Herein, we report that peptide supplementation during Rhizopus oryzae fermentation significantly improved fumaric acid production. Specifically, fumaric acid productivity was elevated by approximately 100%, fermentation duration was shortened from 72 to 36 h, while maintaining the final titer. Furthermore, transcriptome profile analysis and biochemical assays indicated that the overall capabilities of the stress defense systems (enzymatic and nonenzymatic) were significantly improved in R. oryzae. Consequently, glycolytic metabolism was distinctly enhanced, which eventually resulted in improved fumaric acid production and reduced fermentation duration. We expect our findings and efforts to provide essential insights into the optimization of the fermentation performance of filamentous fungi in industrial biotechnology and fermentation engineering.
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Base de dados:
MEDLINE
Assunto principal:
Rhizopus
/
Fumaratos
Idioma:
En
Ano de publicação:
2022
Tipo de documento:
Article