One-step synthesis of well-defined molecularly imprinted nanospheres for the class-selective recognition and separation of ß-blockers in human serum.
J Chromatogr A
; 1673: 463204, 2022 Jun 21.
Article
em En
| MEDLINE
| ID: mdl-35689880
ABSTRACT
ß-blockers are a class of medications that are used to treat abnormal heart rhythms and hypertension. Molecularly imprinted polymers (MIPs) capable of selective recognizing and extracting ß-blockers from complex biological samples hold great promise in bioanalytical and biomedical applications, but developing such artificial receptor materials is still challenging. Herein, we introduce a simple one-step method for the synthesis of well-defined molecularly imprinted nanospheres in high yield (83.6-94.4%) via reversible addition-fragmentation chain transfer (RAFT) precipitation polymerization for the selective recognition and extraction of the ß-blockers from human serum. The prepared MIPs are characterized in terms of morphology, pore properties, binding kinetics, capacity, selectivity, and recognition mechanisms. The uniform nanoscale-imprinted layer favored the rapid mass transfer of ß-blockers. The binding studies showed the high adsorption capacity (126.8 µmol/g) and selectivity of the developed nanomaterial. The investigation on the recognition mechanism reveals that multiple driving forces participate in the binding between MIP and ß-blockers, where hydrogen bonding plays as the dominating role for the specific recognition. The MIP was successfully applied for the direct enrichment of five ß-blockers from human serum with HPLC recoveries ranging from 82.9 to 100.3% and RSD of 0.5-6.9% (n = 3).
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Base de dados:
MEDLINE
Assunto principal:
Impressão Molecular
/
Nanosferas
Limite:
Humans
Idioma:
En
Ano de publicação:
2022
Tipo de documento:
Article