Alterations in B Cell and Follicular T-Helper Cell Subsets in Patients with Acute COVID-19 and COVID-19 Convalescents.

Kudryavtsev, Igor V; Arsentieva, Natalia A; Batsunov, Oleg K; Korobova, Zoia R; Khamitova, Irina V; Isakov, Dmitrii V; Kuznetsova, Raisa N; Rubinstein, Artem A; Stanevich, Oksana V; Lebedeva, Aleksandra A; Vorobyov, Evgeny A; Vorobyova, Snejana V; Kulikov, Alexander N; Sharapova, Maria A; Pevtcov, Dmitrii E; Totolian, Areg A

Kudryavtsev, Igor V; Arsentieva, Natalia A; Batsunov, Oleg K; Korobova, Zoia R; Khamitova, Irina V; Isakov, Dmitrii V; Kuznetsova, Raisa N; Rubinstein, Artem A; Stanevich, Oksana V; Lebedeva, Aleksandra A; Vorobyov, Evgeny A; Vorobyova, Snejana V; Kulikov, Alexander N; Sharapova, Maria A; Pevtcov, Dmitrii E; Totolian, Areg A.

Afiliação

Kudryavtsev IV; Institute of Experimental Medicine, Akademika Pavlova 12, 197376 Saint Petersburg, Russia.
Arsentieva NA; Medical Facutly, First Saint Petersburg State I. Pavlov Medical University, L'va Tolstogo St. 6-8, 197022 Saint Petersburg, Russia.
Batsunov OK; Laboratory of Immunology, Saint Petersburg Pasteur Institute, Mira 14, 197101 Saint Petersburg, Russia.
Korobova ZR; Medical Facutly, First Saint Petersburg State I. Pavlov Medical University, L'va Tolstogo St. 6-8, 197022 Saint Petersburg, Russia.
Khamitova IV; Laboratory of Immunology, Saint Petersburg Pasteur Institute, Mira 14, 197101 Saint Petersburg, Russia.
Isakov DV; Laboratory of Immunology, Saint Petersburg Pasteur Institute, Mira 14, 197101 Saint Petersburg, Russia.
Kuznetsova RN; Laboratory of Immunology, Saint Petersburg Pasteur Institute, Mira 14, 197101 Saint Petersburg, Russia.
Rubinstein AA; Institute of Experimental Medicine, Akademika Pavlova 12, 197376 Saint Petersburg, Russia.
Stanevich OV; Medical Facutly, First Saint Petersburg State I. Pavlov Medical University, L'va Tolstogo St. 6-8, 197022 Saint Petersburg, Russia.
Lebedeva AA; Medical Facutly, First Saint Petersburg State I. Pavlov Medical University, L'va Tolstogo St. 6-8, 197022 Saint Petersburg, Russia.
Vorobyov EA; Laboratory of Immunology, Saint Petersburg Pasteur Institute, Mira 14, 197101 Saint Petersburg, Russia.
Vorobyova SV; Medical Facutly, First Saint Petersburg State I. Pavlov Medical University, L'va Tolstogo St. 6-8, 197022 Saint Petersburg, Russia.
Kulikov AN; Medical Facutly, First Saint Petersburg State I. Pavlov Medical University, L'va Tolstogo St. 6-8, 197022 Saint Petersburg, Russia.
Sharapova MA; Smorodintsev Research Institute of Influenza, Prof. Popov St.15/17, 197376 Saint Petersburg, Russia.
Pevtcov DE; Medical Facutly, First Saint Petersburg State I. Pavlov Medical University, L'va Tolstogo St. 6-8, 197022 Saint Petersburg, Russia.
Totolian AA; Medical Facutly, First Saint Petersburg State I. Pavlov Medical University, L'va Tolstogo St. 6-8, 197022 Saint Petersburg, Russia.

Curr Issues Mol Biol ; 44(1): 194-205, 2021 Dec 30.

Article em En | MEDLINE | ID: mdl-35723393

ABSTRACT

ABSTRACT

BACKGROUND:

Humoral immunity requires interaction between B cell and T follicular helper cells (Tfh) to produce effective immune response, but the data regarding a role of B cells and Tfh in SARS-CoV-2 defense are still sparse.

METHODS:

Blood samples from patients with acute COVID-19 (n = 64), convalescents patients who had specific IgG to SARS-CoV-2 N-protein (n = 55), and healthy donors with no detectable antibodies to any SARS-CoV-2 proteins (HC, n = 44) were analyses by multicolor flow cytometry.

RESULTS:

Patients with acute COVID-19 showed decreased levels of memory B cells subsets and increased proportion plasma cell precursors compared to HC and COVID-19 convalescent patients, whereas for the latter the elevated numbers of virgin naïve, Bm2' and "Bm3+Bm4" was found if compared with HC. During acute COVID-19 CXCR3+CCR6- Tfh1-like cells were decreased and the levels of CXCR3-CCR6+ Tfh17-like were increased then in HC and convalescent patients. Finally, COVID-19 convalescent patients had increased levels of Tfh2-, Tfh17- and DP Tfh-like cells while comparing their amount with HC.