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Intracellular delivery and photothermal therapeutic effects of polyhistidine peptide-modified gold nanoparticles.
Hori, Kosuke; Higashida, Shinichi; Osaki, Tomohiro; Kawano, Tsuyoshi; Inaba, Hiroshi; Matsuura, Kazunori; Iwasaki, Takashi.
Afiliação
  • Hori K; Department of Agriculture, Graduate School of Sustainability Science, Tottori University, Tottori 680-8553, Japan.
  • Higashida S; CDM Infrastructure & Environmental, Inc., Osaka 562-0035, Japan.
  • Osaki T; Joint Department of Veterinary Medicine, Faculty of Agriculture, Tottori University, Tottori 680-8553, Japan.
  • Kawano T; Department of Agriculture, Graduate School of Sustainability Science, Tottori University, Tottori 680-8553, Japan.
  • Inaba H; Department of Chemistry and Biotechnology, Graduate School of Engineering, Tottori University, Tottori 680-8552, Japan.
  • Matsuura K; Department of Chemistry and Biotechnology, Graduate School of Engineering, Tottori University, Tottori 680-8552, Japan.
  • Iwasaki T; Department of Agriculture, Graduate School of Sustainability Science, Tottori University, Tottori 680-8553, Japan. Electronic address: itaka@tottori-u.ac.jp.
J Biotechnol ; 354: 34-44, 2022 Aug 10.
Article em En | MEDLINE | ID: mdl-35724765
Gold nanoparticles (AuNPs) are widely used as an agent in photothermal therapy (PTT) against various cancers. However, a drug delivery system (DDS) is required for effective PTT using AuNPs as AuNPs accumulate passively in tumors. In the present study, we used polyhistidine peptide, a novel cell-penetrating peptide, which is efficiently internalized into tumor cells, as a DDS carrier for PTT using AuNPs. Polyhistidine peptide-modified AuNPs are efficiently internalized into RERF-LC-AI human lung squamous cancer cells and localized to the intracellular lysosome, which is based on the nature of the polyhistidine peptide. Furthermore, the polyhistidine peptide-modified AuNPs inhibited proliferation of RERF-LC-AI cells in a polyhistidine peptide modification-dependent manner under 660 nm laser irradiation. Quantitative real-time PCR showed increased expression levels of an apoptosis-related gene (bax) and heat stress-related gene (hsp70) in RERF-LC-AI cells treated with polyhistidine peptide-modified AuNPs and laser. Our findings highlight the efficacy of AuNPs modified with H16 peptide in PTT.
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Texto completo: 1 Base de dados: MEDLINE Assunto principal: Nanopartículas Metálicas / Peptídeos Penetradores de Células / Histidina Limite: Humans Idioma: En Ano de publicação: 2022 Tipo de documento: Article

Texto completo: 1 Base de dados: MEDLINE Assunto principal: Nanopartículas Metálicas / Peptídeos Penetradores de Células / Histidina Limite: Humans Idioma: En Ano de publicação: 2022 Tipo de documento: Article