Establishment of well-differentiated camelid airway cultures to study Middle East respiratory syndrome coronavirus.
Sci Rep
; 12(1): 10340, 2022 06 20.
Article
em En
| MEDLINE
| ID: mdl-35725865
ABSTRACT
In 2012, Middle East respiratory syndrome coronavirus (MERS-CoV) emerged in Saudi Arabia and was mostly associated with severe respiratory illness in humans. Dromedary camels are the zoonotic reservoir for MERS-CoV. To investigate the biology of MERS-CoV in camelids, we developed a well-differentiated airway epithelial cell (AEC) culture model for Llama glama and Camelus bactrianus. Histological characterization revealed progressive epithelial cellular differentiation with well-resemblance to autologous ex vivo tissues. We demonstrate that MERS-CoV displays a divergent cell tropism and replication kinetics profile in both AEC models. Furthermore, we observed that in the camelid AEC models MERS-CoV replication can be inhibited by both type I and III interferons (IFNs). In conclusion, we successfully established camelid AEC cultures that recapitulate the in vivo airway epithelium and reflect MERS-CoV infection in vivo. In combination with human AEC cultures, this system allows detailed characterization of the molecular basis of MERS-CoV cross-species transmission in respiratory epithelium.
Texto completo:
1
Base de dados:
MEDLINE
Assunto principal:
Camelídeos Americanos
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Infecções por Coronavirus
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Coronavírus da Síndrome Respiratória do Oriente Médio
Limite:
Animals
Idioma:
En
Ano de publicação:
2022
Tipo de documento:
Article