Evaluation of Volumetric Absorptive Microsampling and Mass Spectrometry Data-Independent Acquisition of Hemoglobin-Related Clinical Markers.
J Proteome Res
; 21(8): 1816-1828, 2022 08 05.
Article
em En
| MEDLINE
| ID: mdl-35770882
ABSTRACT
Data-independent acquisition (DIA) allows comprehensive proteome coverage, while it also potentially works as a unified protocol to determine a multitude of proteins found in blood. Because of its high specificity, mass spectrometry may greatly reduce the interference observed in other assays to evaluate blood markers. Here, we combined DIA with volumetric absorptive microsampling (VAMS) and automated proteomics sample processing in a platform to assess clinical markers. As a proof of concept, we evaluated two hemoglobin-related biomarkers the glycated hemoglobin (HbA1c) and hemoglobin (Hb) variants. HbA1c by DIA showed good correlation with the reference method, but method imprecision did not meet the quality requirement for this biomarker. We developed a strategy to identify Hb variants based on a customized database combined with a workflow for DIA data extraction and rigorous peptide evaluation. Data are available via ProteomeXchange with identifier PXD029918.
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Base de dados:
MEDLINE
Assunto principal:
Coleta de Amostras Sanguíneas
/
Teste em Amostras de Sangue Seco
Tipo de estudo:
Guideline
Idioma:
En
Ano de publicação:
2022
Tipo de documento:
Article