Mitochondrial Calcium Handling in Isolated Mitochondria from a Guinea Pig Heart.
Methods Mol Biol
; 2497: 97-106, 2022.
Article
em En
| MEDLINE
| ID: mdl-35771437
ABSTRACT
Mitochondrial calcium (Ca2+) plays a key role in regulating normal cardiac function. A physiological increase in mitochondrial matrix calcium [Ca2+]m drives mitochondrial ATP production to meet the high-energy demands during excitation-contraction coupling. However, a pathological increase in [Ca2+]m leads to increased oxidative stress, impaired bioenergetics, and the opening of mitochondrial permeability transition pore (mPTP), a hallmark of the failing heart. Therefore, a better understanding of the [Ca2+]m handling and its role in heart function and dysfunction is of great importance. Here, we describe a detailed protocol for measuring mitochondrial Ca2+ handling in the isolated functionally intact mitochondria from cardiac tissue of the guinea pig.
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Texto completo:
1
Base de dados:
MEDLINE
Assunto principal:
Cálcio
/
Proteínas de Transporte da Membrana Mitocondrial
Limite:
Animals
Idioma:
En
Ano de publicação:
2022
Tipo de documento:
Article