Production of Human ABC Transporters and Oligosaccharyltransferase Complexes for Structural Studies.

Ramírez, Ana S; Nosol, Kamil; Locher, Kaspar P

Ramírez, Ana S; Nosol, Kamil; Locher, Kaspar P.

Afiliação

Ramírez AS; Institute of Molecular Biology and Biophysics, Eidgenössische Technische Hochschule (ETH), Zürich, Switzerland.
Nosol K; Institute of Molecular Biology and Biophysics, Eidgenössische Technische Hochschule (ETH), Zürich, Switzerland.
Locher KP; Institute of Molecular Biology and Biophysics, Eidgenössische Technische Hochschule (ETH), Zürich, Switzerland. locher@mol.biol.ethz.ch.

Methods Mol Biol ; 2507: 273-294, 2022.

Article em En | MEDLINE | ID: mdl-35773587

Assuntos

Transportadores de Cassetes de Ligação de ATP; Hexosiltransferases; Microscopia Crioeletrônica; Hexosiltransferases/química; Hexosiltransferases/genética; Hexosiltransferases/metabolismo; Humanos; Proteínas de Membrana/metabolismo

Palavras-chave

ABC transporters; Cryo-EM; Human membrane proteins; In vitro glycosylation assays; Oligosaccharyltransferase; Production; Purification; Reconstitution in nanodiscs

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Texto completo: 1 Base de dados: MEDLINE Assunto principal: Transportadores de Cassetes de Ligação de ATP / Hexosiltransferases Limite: Humans Idioma: En Ano de publicação: 2022 Tipo de documento: Article

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