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Dual-mode chemosensor for the fluorescence detection of zinc and hypochlorite on a fluorescein backbone and its cell-imaging applications.
Maity, Sibaprasad; Maity, Annada C; Das, Avijit Kumar; Bhattacharyya, Nandan.
Afiliação
  • Maity S; Department of Applied Sciences, Haldia Institute of Technology, Hatiberia, Haldia 721657, West Bengal, India. spmaity2003@gmail.com.
  • Maity AC; Department of Chemistry, Indian Institute of Engineering Science and Technology, Shibpur, Howrah 711103, West Bengal, India.
  • Das AK; Department of Chemistry, CHRIST (Deemed to be University), Hosur Road, Bengaluru, Karnataka, 560029, India. avijitkumar.das@christuniversity.in.
  • Bhattacharyya N; Panskura Banamali College, Purba Medinipur, West Bengal, India.
Anal Methods ; 14(28): 2739-2744, 2022 07 21.
Article em En | MEDLINE | ID: mdl-35775440
Fluorescein coupled with 3-(aminomethyl)-4,6-dimethylpyridin-2(1H)-one (FAD) was synthesized for the selective recognition of Zn2+ over other interfering metal ions in acetonitrile/aqueous buffer (1 : 1). Interestingly, there was a significant fluorescence enhancement of FAD in association with Zn2+ at 426 nm by strong chelation-induced fluorescence enhancement (CHEF) without interrupting the cyclic spirolactam ring. A binding stoichiometric ratio of 1 : 2 for the ligand FAD with metal Zn2+ was proven by a Jobs plot. However, the cyclic spirolactam ring was opened by hypochlorite (OCl-) as well as oxidative cleavage of the imine bond, which resulted in the emission enhancement of the wavelength at 520 nm. The binding constant and detection limit of FAD towards Zn2+ were determined to be 1 × 104 M-1 and 1.79 µM, respectively, and the detection limit for OCl- was determined as 2.24 µM. We introduced here a dual-mode chemosensor FAD having both the reactive functionalities for the simultaneous detection of Zn2+ and OCl- by employing a metal coordination (Zn2+) and analytes (OCl-) induced chemodosimetric approach, respectively. Furthermore, for the practical application, we studied the fluorescence imaging inside HeLa cells by using FAD, which demonstrated it can be very useful as a selective and sensitive fluorescent probe for zinc.
Assuntos

Texto completo: 1 Base de dados: MEDLINE Assunto principal: Zinco / Ácido Hipocloroso Tipo de estudo: Diagnostic_studies Limite: Humans Idioma: En Ano de publicação: 2022 Tipo de documento: Article

Texto completo: 1 Base de dados: MEDLINE Assunto principal: Zinco / Ácido Hipocloroso Tipo de estudo: Diagnostic_studies Limite: Humans Idioma: En Ano de publicação: 2022 Tipo de documento: Article