Optimized in vitro three-dimensional invasion assay for quantifying a wide range of cancer cell invasive behavior.
STAR Protoc
; 3(3): 101516, 2022 09 16.
Article
em En
| MEDLINE
| ID: mdl-35780429
ABSTRACT
We describe a three-dimensional (3D) in vitro assay for quantifying cancer cell invasion into a 3D microenvironment with defined biochemical and biophysical properties. Researchers can quantify invasion dynamics (e.g., cell motility and directionality) and examine morphological changes during invasion, using live-cell and confocal imaging techniques. Together, these advantages over existing in vitro invasion assays, such as transwell-based assays, provide researchers with a valuable tool to gain insight into the mechanisms regulating cancer cell invasion. For complete details on the use and execution of this protocol, please refer to Padilla-Rodriguez et al. (2018) and Watson et al. (2021).
Palavras-chave
Texto completo:
1
Base de dados:
MEDLINE
Assunto principal:
Microambiente Tumoral
Limite:
Humans
Idioma:
En
Ano de publicação:
2022
Tipo de documento:
Article