Your browser doesn't support javascript.
loading
A fluorescent probe for monitoring sulfite in living cells with large Stokes shift and rapid response.
Li, Qin; Zhou, Kang-Min; Wang, Bin; Wang, Bao-Zhong; Yang, Yu-Shun; Zhu, Hai-Liang.
Afiliação
  • Li Q; State Key Laboratory of Pharmaceutical Biotechnology, School of Life Sciences, Nanjing University, Nanjing, 210023, China.
  • Zhou KM; State Key Laboratory of Pharmaceutical Biotechnology, School of Life Sciences, Nanjing University, Nanjing, 210023, China.
  • Wang B; State Key Laboratory of Pharmaceutical Biotechnology, School of Life Sciences, Nanjing University, Nanjing, 210023, China.
  • Wang BZ; State Key Laboratory of Pharmaceutical Biotechnology, School of Life Sciences, Nanjing University, Nanjing, 210023, China. Electronic address: wbz@nju.edu.cn.
  • Yang YS; State Key Laboratory of Pharmaceutical Biotechnology, School of Life Sciences, Nanjing University, Nanjing, 210023, China; Jinhua Advanced Research Institute, Jinhua, 321019, China. Electronic address: ys_yang@nju.edu.cn.
  • Zhu HL; State Key Laboratory of Pharmaceutical Biotechnology, School of Life Sciences, Nanjing University, Nanjing, 210023, China. Electronic address: zhuhl@nju.edu.cn.
Anal Biochem ; 654: 114800, 2022 10 01.
Article em En | MEDLINE | ID: mdl-35792183
Sulfite (SO32-) is considered as a monitor of a wide range of physiological processes. However, cells and tissues are adversely affected when the body ingests high level of sulfite. Here, we designed and synthesized a "turn on" fluorescent probe ImiSft-1 with 2-cyano-N-methylacetamide as the specific recognition site of SO32-. This probe predominantly achieved high response intensity to SO32- and desirable properties such as large Stokes shift (∼180 nm), fast response time (within 15 s), and high sensitivity (LOD = 0.12 µM). Importantly, the probe was highly selective for sulfite from other bio-species including biological thiols. Other functional properties included broad pH adaptability (5.0-10.0) and low cytotoxicity. Given these advantages and the fluorescence imaging in living MCF-7 cells, it was demonstrated that probe ImiSft-1 could monitor the changes of sulfite concentration in living cells.
Assuntos
Palavras-chave

Texto completo: 1 Base de dados: MEDLINE Assunto principal: Sulfitos / Corantes Fluorescentes Limite: Humans Idioma: En Ano de publicação: 2022 Tipo de documento: Article

Texto completo: 1 Base de dados: MEDLINE Assunto principal: Sulfitos / Corantes Fluorescentes Limite: Humans Idioma: En Ano de publicação: 2022 Tipo de documento: Article