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Long Non-coding RNA UCA1 Regulates SRPK1 Expression Through miR- 99b-3p in Ovarian Cancer.
Xu, Juan; Zheng, Liu-Hong; Hong, Yi-Nuo; Xuan, Cheng; Yan, Shu-Ling; Lv, Guo-Liang; Jiang, Zheng-Gang; Ding, Xian-Feng.
Afiliação
  • Xu J; College of Life Sciences and Medicine, Zhejiang Sci-Tech University, Hangzhou, Zhejiang 310018, China.
  • Zheng LH; College of Life Sciences and Medicine, Zhejiang Sci-Tech University, Hangzhou, Zhejiang 310018, China.
  • Hong YN; College of Life Sciences and Medicine, Zhejiang Sci-Tech University, Hangzhou, Zhejiang 310018, China.
  • Xuan C; College of Life Sciences and Medicine, Zhejiang Sci-Tech University, Hangzhou, Zhejiang 310018, China.
  • Yan SL; College of Life Sciences and Medicine, Zhejiang Sci-Tech University, Hangzhou, Zhejiang 310018, China.
  • Lv GL; Library of Zhejiang Sci-Tech University, Hangzhou, Zhejiang 310018, China.
  • Jiang ZG; Department of Science Research and Information and Management, Zhejiang Provincial Centers for Disease Control and Prevention, Hangzhou, Zhejiang 310051, China.
  • Ding XF; College of Life Sciences and Medicine, Zhejiang Sci-Tech University, Hangzhou, Zhejiang 310018, China.
Protein Pept Lett ; 29(10): 829-838, 2022.
Article em En | MEDLINE | ID: mdl-35794743
BACKGROUND: Ovarian carcinoma (OC) is one of the most common malignancies of the female reproductive organs, with a low survival rate primarily due to the lack of effective methods for early diagnosis and prognosis. OBJECTIVE: In this article, our motivation is to explore the lncRNA-related network mechanisms involved in the pathogenesis of OC. METHODS: Public lncRNAs and mRNA expression datasets for OC were collected from the Gene Expression Omnibus (GEO) database. By integrated bioinformatics analysis, we constructed a UCA1-miRNA-mRNA network. We studied lncRNA-related molecular modulation mechanism in ovarian cancer cells based on MTT assay, dual luciferase reporter gene assays, quantitative realtime PCR, and western blotting. RESULTS: UCA1 was higher in ovarian tumor tissues and cells than normal tissues and cells. It was demonstrated in this study that knockdown of UCA1 inhibited ovarian cancer cell viability, which a miR-99b-3p inhibitor could reverse in vitro. Further, UCA1 was shown to regulate the expression of SRPK1 by directly binding to miR-99b-3p. CONCLUSION: These results suggest that UCA1 functions as an oncogene in ovarian cancer. Inhibition of UCA1/miR-99b-3p/SRPK1 axis may become a novel target for treating ovarian cancer.
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Texto completo: 1 Base de dados: MEDLINE Assunto principal: Neoplasias Ovarianas / MicroRNAs / RNA Longo não Codificante Tipo de estudo: Screening_studies Limite: Female / Humans Idioma: En Ano de publicação: 2022 Tipo de documento: Article

Texto completo: 1 Base de dados: MEDLINE Assunto principal: Neoplasias Ovarianas / MicroRNAs / RNA Longo não Codificante Tipo de estudo: Screening_studies Limite: Female / Humans Idioma: En Ano de publicação: 2022 Tipo de documento: Article