Transformation of yeast by a replicating hybrid plasmid.
Nature
; 275(5676): 104-9, 1978 Sep 14.
Article
em En
| MEDLINE
| ID: mdl-357984
ABSTRACT
Chimaeric plasmids have been constructed containing a yeast plasmid and fragments of yeast nuclear DNA linked to pMB9, a derivative of the ColEl plasmid from E. coli. Two plasmids were isolated which complement leuB mutations in E. coli. These plasmids have been used to develop a method for transforming a leu2 strain of S. cerevisiae to Leu+ with high frequency. The yeast transformants contained multiple plasmid copies which were recovered by transformation in E. coli. The yeast plasmid sequence recombined intramolecularly during propagation in yeast.
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Base de dados:
MEDLINE
Assunto principal:
Plasmídeos
/
Saccharomyces cerevisiae
/
Transformação Genética
/
DNA Recombinante
/
Escherichia coli
Idioma:
En
Ano de publicação:
1978
Tipo de documento:
Article