Full Assessment of Lung Mechanics Using Computer-Controlled, Forced Oscillation Technique.
Curr Protoc
; 2(7): e488, 2022 Jul.
Article
em En
| MEDLINE
| ID: mdl-35834677
ABSTRACT
The forced oscillation technique (FOT) is a powerful and accurate method to quantify the mechanical properties of the airways and tissues of the respiratory system. Here we provide a detailed protocol for the measurement of mouse respiratory mechanical parameters. We present a procedure for mouse endotracheal intubation using a handcrafted intubation platform and confirmation module. The FlexiVentFX™ system (Scireq Inc.) is utilized for the thorough assessment of lung function with the FlexiWare™ software serving as a unit for the planning, experimentation, and analysis. The protocol has been standardized and adapted for use by our center for lung-function phenotyping of mouse models generated for the International Mouse Phenotyping Consortium (IMPC). The simplified steps, technical considerations, and integrated hardware-software demonstration make this protocol adaptable and implementable for researchers interested in using FOT for lung-function evaluation. © 2022 The Authors. Current Protocols published by Wiley Periodicals LLC. Support Protocol 1 Assembly of the FlexiVentFX™ system for measurements Support Protocol 2 FlexiWare database management Support Protocol 3 A guide for the construction of intubation platform and confirmation module Basic Protocol 1 Mouse endotracheal intubation Basic Protocol 2 Assessment of mouse basal lung function.
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Texto completo:
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Base de dados:
MEDLINE
Assunto principal:
Mecânica Respiratória
/
Pulmão
Limite:
Animals
Idioma:
En
Ano de publicação:
2022
Tipo de documento:
Article