Production of a high molecular weight levan by Bacillus paralicheniformis, an industrially and agriculturally important isolate from the buffalo grass rhizosphere.
Antonie Van Leeuwenhoek
; 115(9): 1101-1112, 2022 Sep.
Article
em En
| MEDLINE
| ID: mdl-35840814
ABSTRACT
A new exopolysaccharide (EPS) producing Gram-positive bacterium was isolated from the rhizosphere of Bouteloua dactyloides (buffalo grass) and its EPS product was structurally characterized. The isolate, designated as LB1-1A, was identified as Bacillus paralicheniformis based on 16S rRNA gene sequence and phylogenetic tree analysis. The EPS produced by LB1-1A was identified as a levan, having ß(2 â 6) linked backbone with ß(2 â 1) linkages at the branch points (4.66%). The isolate LB1-1A yielded large amount (~ 42 g/l) of levan having high weight average molecular weight (Mw) of 5.517 × 107 Da. The relatively low degree of branching and high molecular weight of this levan makes B. paralicheniformis LB1-1A a promising candidate for industrial applications.
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Base de dados:
MEDLINE
Assunto principal:
Rizosfera
/
Frutanos
Idioma:
En
Ano de publicação:
2022
Tipo de documento:
Article