Polysaccharide from Echinacea purpurea plant ameliorates oxidative stress-induced liver injury by promoting Parkin-dependent autophagy.
Phytomedicine
; 104: 154311, 2022 Sep.
Article
em En
| MEDLINE
| ID: mdl-35843188
ABSTRACT
BACKGROUND:
Acetaminophen (APAP) overdose represents one of the most common drug-induced liver injuries (DILI) worldwide. Oxidative damage to the hepatocytes and their resultant autophagy are the key components in the APAP-induced DILI. Echinacea purpurea polysaccharide (EPPS), the component extracted from the root of Echinacea purpurea (L.) Moench, shows various biological functions including immunoregulation and antioxidant activity.PURPOSE:
This study aimed to elucidate the protective effect of EPPS against APAP-induced DILI and the underlying mechanisms.RESULTS:
EPPS attenuates APAP overdose induced DILI in mice and ameliorates inflammation and oxidative stress in mice with APAP overdose-induced DILI. Furthermore, EPPS protected the hepatocytes against APAP-induced liver injury by suppressing apoptosis. EPPS ameliorates APAP-induced DILI via an autophagy-dependent mechanism in vivo and increases autophagy with a reduction in oxidative stress and inflammation in vitro. Parkin knockdown prevents the autophagic-dependent manner of EPPS effects in APAP-treated hepatocytes.CONCLUSIONS:
EPPS exhibited a strong hepatoprotective effect against APAP-induced DILI and was correlated with reduction of autophagy-dependent oxidant response, inflammation, and apoptosis. Moreover, the findings indicated that EPPS exerts its hepatoprotective effect against APAP mainly via Parkin-dependent autophagy, and the use of EPPS can serve as a promising novel therapeutic strategy for APAP-induced DILI.Palavras-chave
Texto completo:
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Base de dados:
MEDLINE
Assunto principal:
Echinacea
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Doença Hepática Induzida por Substâncias e Drogas
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Doença Hepática Crônica Induzida por Substâncias e Drogas
Limite:
Animals
Idioma:
En
Ano de publicação:
2022
Tipo de documento:
Article