[Regulation of vascular endothelial cell activator expression by tanshinone â
¡_A based on PI3K/Akt signaling pathway].
Zhongguo Zhong Yao Za Zhi
; 47(13): 3589-3596, 2022 Jul.
Article
em Zh
| MEDLINE
| ID: mdl-35850813
ABSTRACT
The present study investigated the regulatory effect of tanshinone â
¡_A(TAâ
¡_A) on activator expression in human umbilical vein endothelial cells(HUVECs) and the effect on the phosphoinositide 3-kinase(PI3 K)/protein kinase B(Akt) signaling pathway in patients with antiphospholipid syndrome(APS). HUVECs cultured in vitro were divided into a medium group, a blank control group, an APS model group, an APS+LY5 group, an APS+LY10 group, an APS+LY20 group, an APS+TAâ
¡_A5 group, an APS+TAâ
¡_A10 group, an APS+TAâ
¡_A20 group, and an APS+TAâ
¡_A10+LY10 group. The effects of LY294002 and TAâ
¡_A at different concentrations on the secretion of interleukin-6(IL-6), interleukin-8(IL-8), and monocyte chemoattractant protein-1(MCP-1) by HUVECs were investigated. The effects on the mRNA expression of annexin A2(ANXA2), PI3 K, Akt, and E-cadherin(E-cad) were detected by quantitative polymerase chain reaction(qPCR), and Western blot was used to determine the effects on the protein expression of ANXA2, p-PI3 K/PI3 K, p-Akt/Akt, and E-cad. The results revealed that compared with the APS model group, the APS+TAâ
¡_A10 group showed statistically reduced IL-6 and MCP-1 and increased IL-8 in a concentration-dependent manner with the increase in TAâ
¡_A dose, while the APS+TAâ
¡_A10 group showed increased mRNA and protein expression of ANXA2, PI3 K, Akt, and E-cad(P<0.05 or P<0.01) in a concentration-dependent manner with the increase in TAâ
¡_A dose. The findings indicated that the serum of APS patients could lead to the decreased mRNA and protein expression levels of ANXA2, PI3 K, Akt, and E-cad in HUVECs, increased secretion of IL-6 and MCP-1, and reduced secretion of IL-8, and activate vascular endothelial cells. In contrast, once the PI3 K/Akt signaling pathway was blocked, the mRNA and protein expression of ANXA2 and E-cad significantly decreased, IL-6 and MCP-1 secretion significantly increased, and IL-8 secretion was significantly reduced. It suggests that TAâ
¡_A regulates the activation of vascular endothelial cells in APS patients by activating the PI3 K/Akt signaling pathway.
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Texto completo:
1
Base de dados:
MEDLINE
Assunto principal:
Fosfatidilinositol 3-Quinases
/
Proteínas Proto-Oncogênicas c-akt
Tipo de estudo:
Prognostic_studies
Limite:
Humans
Idioma:
Zh
Ano de publicação:
2022
Tipo de documento:
Article