Lutidine derivatives for live-cell imaging of the mitochondria and endoplasmic reticulum.
Org Biomol Chem
; 20(35): 7047-7055, 2022 09 14.
Article
em En
| MEDLINE
| ID: mdl-35851393
ABSTRACT
The mitochondria and endoplasmic reticulum (ER) are highly dynamic subcellular structures essential for several biological functions. The development of non-toxic, wash-free fluorophores to visualize these structures inside cells aid in understanding their localization and dynamics in diverse cellular processes. In this paper, we report the synthesis and characterization of lutidine-based cationic fluorophores bearing push-pull substituents exhibiting emission in green and red wavelength regions and their subcellular localization in living cells. The confocal imaging of the molecules in a cellular domain reveals the robust localization of three molecules (2, 4 and 5) in the mitochondria and two molecules with polyfluorophenyl substituents (6 and 7) in the ER. At the same time, the other two molecules (1 and 3) showed non-specific imaging. These molecules can also be used to sense the altered viscosity of the stressed ER and investigate its dynamics.
Texto completo:
1
Base de dados:
MEDLINE
Assunto principal:
Retículo Endoplasmático
/
Mitocôndrias
Idioma:
En
Ano de publicação:
2022
Tipo de documento:
Article